National Node of the BCH
Modified Organism
ACS-BNØØ7-1 - Liberty-Link™ Innovator Canola
Record information and status
Record ID
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2017-06-05 14:16 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-06-05 14:16 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
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LMO name
Liberty-Link™ Innovator Canola
Transformation event
Topas 19/2 (HCN92)
Unique identifier
Glyphosate herbicide tolerant canola produced by inserting the phosphinothricin acetyltransferase (pat) gene conferring tolerance to Phosphinothricin (Glufosinate ammonium) herbicide and neomycin phosphotransferase II (npt II) conferring resistance to the herbicide kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
ACS-BNØØ8-2 - Herbicide Tolerant Canola
Resistance to herbicides - Glufosinate
Show detection method(s)
Liberty Link™ Independence™ Canola
Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
CaMV 35S promoter
0.53 Kb
Phosphinothricin N-acetyltransferase gene
0.55 Kb
CaMV 35S terminator
0.22 Kb
Nopaline Synthase Gene Promoter
0.34 Kb
Neomycin Phosphotransferase II
0.80 Kb
Octopine Synthase Gene Terminator
0.22 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Southern blot analysis indicated that 2 copies of the T-DNA cassette was transformed into the host genome at a single insertion site. The 2 cassettes were arranged in an inverted RB/RB arrangement. Furthermore one of the two nptII genes, one was only partially integrated.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The canola line HCN92 was genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.

Glufosinate tolerance in HCN92 is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound.
Other relevant website address or attached documents