National Node of the BCH
Modified Organism
SYN-EV176-9 - NaturGard KnockOut™ maize
Record information and status
Record ID
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-08-14 18:09 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-14 18:09 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
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LMO name
NaturGard KnockOut™ maize
Transformation event
Bt176 (176)
Unique identifier
This LMO contains two copies of a truncated synthetic version of the full length cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. The synthetic truncated cry1Ab gene encodes a protein that corresponds to the first 648 amino acids of the N-terminal of the 1155 amino acid full length native Cry1Ab protein and includes the portion of the native protein that is necessary for insect control.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Proprietary Ciba Seeds inbred maize line CG00526
Characteristics of the transformation process
pCIB3064 and pCIB4431
Techniques used for the modification
  • Biolistic / Particle gun
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Phosphinothricin N-acetyltransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to herbicides - Glufosinate
Cry1Ab - Bacillus thuringiensis - Bt, Bacillus, BACTU
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Beta-lactamase gene - Escherichia coli - ECOLX
Resistance to antibiotics - Ampicillin
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
CaMV 35S terminator - Cauliflower mosaic virus - CaMV
Phosphoenolpyruvate Carboxylase gene promoter - Zea mays - Maize, Corn, MAIZE
Calcium-dependent protein kinase promoter - Zea mays - Maize, Corn, MAIZE
Phosphoenolpyruvate carboxylase, intron 9 - Zea mays - Maize, Corn, MAIZE
Notes regarding the genetic elements introduced or modified in this LMO
Additional information concerning the cry1Ab gene inserts in this LMO:
The expression of the two copies of the cry1Ab genes are under the control either of a pollen-specific promoter the from a calcium-dependent protein kinase or green tissue-specific promoter phosphoenolpyruvate carboxylase. Both promoters were isolated from maize. The termination sequences for both of genes was from cauliflower mosaic virus (CaMV), a known plant pest.

Additional information concerning the bar gene insert in this LMO:
This LMO contains one copy of the bar gene from Streptomyces hygroscopicus which encodes for phosphinotricin acetyltransferase (PAT) that confers resistance to glufosinate herbicide. The bar gene is under the regulation of the 35S promoter and the 35S terminator from the cauliflower mosaic virus (CaMV).

Additional information concerning the bla gene insert in this LMO:
The bla gene from Escherichia coli is not expressed in plant cells, but was employed as a selectable trait for screening bacterial colonies for the presence of the plasmid vector.

Additional information on the inserted genetic material:

Two plasmids, pCIB3064 and pCIB4431 were used as vectors for the transformation of Bt176 maize. Both are derivatives of the plasmid PUC18, which has a molecular weight of 2.7 kb and contains sequences such as prokaryotic gene bla and gene lacZ. 

Plasmid pCIB3064 contains one copy of the bar gene which is under the regulation of the 35S promoter and the 35S terminator from the cauliflower mosaic virus (CaMV).  The plasmid pCIB4431 contains two copies of a synthetic truncated cry1Ab gene; the first copy which is under the regulation of a promoter derived from maize phosphoenolpyruvate carboxylase gene and the CaMV 35S terminator; and the second copy which is under the regulation of a promoter derived from maize calcium-dependent protein kinase gene ("pollen promoter') and the CaMV 35S terminator.

There are uncertainties regarding the copy number of the inserts in Bt176. Evidence suggests that 2-5 copies of the inserts may be present (see document below on the molecular characterization of Bt176).
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information
Other relevant website address or attached documents