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National BCH > Record details
Modified Organism
MON-ØØ6Ø3-6 x ACS-ZMØØ3-2 - Roundup Ready™ Liberty Link™ maize
Record information and status
Record ID
100975
Status
Published
Date of creation
2010-01-29 01:42 UTC (bch@env.go.jp)
Date of last update
2013-07-24 16:28 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 16:28 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ Liberty Link™ maize
Transformation event
NK603 x T25
Unique identifier
MON-ØØ6Ø3-6 x ACS-ZMØØ3-2
Developer(s)
Monsanto Company
800 N. Lindbergh Blvd.
St. Louis, MO 63167, USA
Phone:+1 314 694-1000
Url:Monsanto webpage
Description
The stacked maize line NK603 x T25 was produced by cross breeding between maize LMOs T25 and NK603 for tolerance to herbicides containing glufosinate and glyphosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
ACS-ZMØØ3-2 - Liberty Link™ maize
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMGT32 and pDH51
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
Beta-lactamase gene
0.86 Kb
 
 
pUC origin of replication
2.63 Kb
 
 
CaMV 35S promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.53 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from NK603 vector PV-ZMGT32:

NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.

DNA insert from T25 vector pDH51:

Glufosinate tolerance in T25 maize is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Detection method(s)
Additional information
NK603 x T25 was produced through cross breeding and contains the inserted genetic elements from both parents.

From the NK603 parent, NK603 x T25 contains one copy of a DNA cassette containing two cp4 epsps genes (see below). From the T25 parent, NK603 x T25 contains one copy of the DNA cassette with an intact Ori-pUC and the "P-35S - pat - T-35S" construct as well as a truncated bla gene (25% of the 5' end of the bla gene is missing).
Additional Information
Other relevant website address or attached documents
Convention on Biological Diversity | Central Portal of the Biosafety Clearing-House